THE ROLE OF INTERLEUKIN 10 (rs1800872) AND HEAT SHOCK PROTEIN 70-2 (rs1061581) GENES IN THE MECHANISMS OF COLIENTERITIS DEVELOPMENT
Abstract
THE ROLE OF INTERLEUKIN 10 (rs1800872) AND HEAT SHOCK PROTEIN 70-2 (rs1061581) GENES IN THE MECHANISMS OF COLIENTERITIS DEVELOPMENT (Abstract): E. coli is one of the commonest causes of diarrhea worldwide responsible for 20-50% of all infectious diarrheas. Genetic background of the condition, including the possible role of IL-10 (rs1800872) and thermal shock protein 70-2 (rs1061581) genes remains unclear. Aim: To find the possible role of IL-10 (rs1800872) and Heat Shock Protein (HSP) 70-2 (rs1061581) genes in the mechanisms of colienteritis pathogenesis. Material and methods: 95 patients with diarrheal escherichiosis and 30 practically healthy individuals (control) participated in the study. Gender composition presented with 62 women (65.26%) and 33 men (34.74%), mean age – 38.66±3.11 yrs. The single nucleotide polymorphisms (SNP) of HSP70-2 (A1267G) and IL-10 (C-592A) genes were studied in PCR. Results: Among patients, IL-10 (rs1800872) and HSP70-2 (rs1061581) genetic mutations were observed with frequencies of 9.47% and 3.16%, respectively, compared to absence in control. Allelic distribution of HSP70-2 gene's 1267G>A SNP showed 2.62 times G-allele domination over A-allele (р<0.001): among patients by 43.16% (c2=70.78; р<0.001), and in control by 50.0% (c2=30.0; р<0.001), with insignificant excessive heterozygosity, not disturbing expected Hardy-Weinberg's equilibrium. Allelic distribution of IL-10 gene's C-592A SNP shows C-allele 2.9 times dominated (р<0.001) in general population: among patients by 43.16% (c2=70.78; р<0.001), in control – by 66.66% (c2=53.33; р<0.001), with preserved population equilibrium. Conclusions: Mutant А-allele of the IL-10 (rs1800872) gene associates with almost twice increased colienteritis risk in observed population [OR=1.98; 95%CI OR=1.0-4.20; р=0.046], with protective role of wild С-allele [OR=0.50; 95%CI OR=0.24-1.01; р=0.058]. HSP70-2 gene's SNP (rs1061581) does not associate with colienteritis risk.
References
2. ***US Burden of Disease Collaborators. The State of US Health, 1990-2016: Burden of Diseases, Injuries, and Risk Factors among US States. JAMA 2018; 319(14): 1-33.
3. ***GBD 2015 Eastern Mediterranean Region Diarrhea Collaborators. Burden of diarrhea in the Eastern Mediterranean Region, 1990-2015: Findings from the Global Burden of Disease 2015 study. Int J Public Health 2018; 63(Suppl 1): 109.
4. Desilets M, Deng Xi, Rao C, et al. Genome-based Definition of an Inflammatory Bowel Disease-associated Adherent-Invasive Escherichia coli Pathovar. Inflamm Bowel Dis 2016; 22(1): 1-12.
5. Sydorchuk L, Yarynych Y, Sydorchuk A, et al. Hepatocytes' function and adipokines in patients with non-alcoholic fatty liver disease depending on the ACE (rs4646994) and PPAR-2 (rs1801282) genes' polymorphisms. Rev Med Chir Soc Med Nat Iaşi 2018; 122(2): 358-364.
6. Sydorchuk LP, Sokolenko AA, Sydorchuk AR, et al. Insulin resistance in patients with arterial hyper-tension and abdominal obesity depending on ACE and PPAR-γ2 genes polymorphism: a new opinion concerning an old problem. The New Armenian Medical J 2015; 9(2): 43-51.
7. Sydorchuk LP, Serdulets YuI, Sydorchuk AR, et al. The polymorphism of matrilin-3 (rs77245812) and interleukin-10 (rs1800872) genes in osteo-arthritis patients with arterial hypertension, obesity and type 2 diabetes mellitus. Archives of the Balkan Medical Union 2017; 52(4): 422-429.
8. Sydorchuk L, Bochkariova O, Andriyets O, Sydorchuk A, Bodnariuk O. Heat-shock protein 70-2 gene (1267A→G) in association with vulvovaginitis and cytokines levels in juvenile girls of Northern Bukovina. The Pharma Innovation Journal 2016; 5(3): 19-23.
9. Wischmeyer PE, Musch MW, Madonna MB, Thisted R, Chang EB. Glutamine protects intestinal epithelial cells: role of inducible HSP70. Am J Physiol 1997; 272 (4 Pt 1): G879-884.
10. Tahara T, Shibata T, Arisawa T, et al. The BB genotype of heat-shock protein (HSP) 70-2 gene is associated with gastric pre-malignant condition in H. pylori-infected older patients. Anticancer Res 2009; 29(8): 3453-3458.
11. Tahara T, Arisawa T, Shibata T, et al. Role of heat-shock protein (HSP) 70-2 genotype in peptic ulcer in Japanese population. Hepatogastroenterology 2012; 59(114): 426-429.
12. Lin JA, Li WQ, Ye DF, et al. The heat shock protein 70-2 gene +1267(A/G) polymorphism and susceptibility to ankylosing spondylitis in Chinese Han population. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2007; 24(5): 574-575.
13. Tahara T, Shibata T, Okubo M, et al. Heat-shock protein 70-2 BB genotype is associated with reduced risks of the steroid-dependent and refractory phenotypes of ulcerative colitis. Biomed Rep 2014; 2(4): 555-558.
14. Karaca E, Kayikcioglu M, Onay H, et al. The effect of interleukin-10 gene promoter polymorphisms on early-onset coronary artery disease. The Anatolian Journal of Cardiology 2011; 11(4): 285-289.
15. Grant AV, Araujo MI, Ponte EV, et al. Polymorphisms in IL-10 are associated with total Immuno-globulin E level and Schistosoma mansoni infection intensity in a Brazilian population. Genes and Immunity 2011; 12: 46-50.
16. Alvarado-Arnez LE, Amaral EP, Sales-Marques C, et al. Association of IL-10 Polymorphisms and Leprosy: A Meta-Analysis. PlosOne 2015; 10(9): e0136282.
17. Sofian M, Kalantar E, Aghakhani A, et al. No correlation between Interleukin-10 Gene Promoter Polymorphisms and Hepatitis B virus infection Outcome. Hepatitis Mon 2013; 13(5): e8803.
18. Valença SPC, Vieira da SA, Nascimento ST, et al. There is no evident correlation between interleukin-10 gene polymorphisms and periportal fibrosis regression after specific treatment. Revista da Sociedade Brasileira de Medicina Tropical 2016; 49(6): 781-785.
Additional Files
Published
Issue
Section
License
COPYRIGHT
Once an article is accepted for publication, MSJ requests a transfer of copyrights for published articles.
COPYRIGHT TRANSFER FORM FOR
REVISTA MEDICO-CHIRURGICALĂ A SOCIETĂȚII DE MEDICI ȘI NATURALIȘTI DIN IAȘI /
THE MEDICAL-SURGICAL JOURNAL OF THE SOCIETY OF PHYSICIANS AND NATURALISTS FROM IASI
We, the undersigned authors of the manuscript entitled
_____________________________________________________________________________________
_____________________________________________________________________________________
warrant that this manuscript, which is submitted for publication in the REVISTA MEDICO-CHIRURGICALĂ, has not been published and it is not under consideration for publication in another journal.
- we give the consent for publication in the REVISTA MEDICO-CHIRURGICALĂ, in printed and electronic format and we transfer unconditioned and complete the copyright of this manuscript to the REVISTA MEDICO-CHIRURGICALĂ, in the event of its acceptance.
- the manuscript does not break the intellectual property rights of any other person.
- we have read the submitted version of the manuscript and we are fully responsible for the content.
Names and signatures of authors / copyright owners (the following sequence is the authorship of the article):
- ______________________________/_________________________
- ______________________________/_________________________
- ______________________________/_________________________
N.B. All the authors must sign this form