THE ROLE OF INTERLEUKIN 10 (rs1800872) AND HEAT SHOCK PROTEIN 70-2 (rs1061581) GENES IN THE MECHANISMS OF COLIENTERITIS DEVELOPMENT
Abstract
THE ROLE OF INTERLEUKIN 10 (rs1800872) AND HEAT SHOCK PROTEIN 70-2 (rs1061581) GENES IN THE MECHANISMS OF COLIENTERITIS DEVELOPMENT (Abstract): E. coli is one of the commonest causes of diarrhea worldwide responsible for 20-50% of all infectious diarrheas. Genetic background of the condition, including the possible role of IL-10 (rs1800872) and thermal shock protein 70-2 (rs1061581) genes remains unclear. Aim: To find the possible role of IL-10 (rs1800872) and Heat Shock Protein (HSP) 70-2 (rs1061581) genes in the mechanisms of colienteritis pathogenesis. Material and methods: 95 patients with diarrheal escherichiosis and 30 practically healthy individuals (control) participated in the study. Gender composition presented with 62 women (65.26%) and 33 men (34.74%), mean age – 38.66±3.11 yrs. The single nucleotide polymorphisms (SNP) of HSP70-2 (A1267G) and IL-10 (C-592A) genes were studied in PCR. Results: Among patients, IL-10 (rs1800872) and HSP70-2 (rs1061581) genetic mutations were observed with frequencies of 9.47% and 3.16%, respectively, compared to absence in control. Allelic distribution of HSP70-2 gene's 1267G>A SNP showed 2.62 times G-allele domination over A-allele (р<0.001): among patients by 43.16% (c2=70.78; р<0.001), and in control by 50.0% (c2=30.0; р<0.001), with insignificant excessive heterozygosity, not disturbing expected Hardy-Weinberg's equilibrium. Allelic distribution of IL-10 gene's C-592A SNP shows C-allele 2.9 times dominated (р<0.001) in general population: among patients by 43.16% (c2=70.78; р<0.001), in control – by 66.66% (c2=53.33; р<0.001), with preserved population equilibrium. Conclusions: Mutant А-allele of the IL-10 (rs1800872) gene associates with almost twice increased colienteritis risk in observed population [OR=1.98; 95%CI OR=1.0-4.20; р=0.046], with protective role of wild С-allele [OR=0.50; 95%CI OR=0.24-1.01; р=0.058]. HSP70-2 gene's SNP (rs1061581) does not associate with colienteritis risk.
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