MORUSIN AND KUWANON G – PROMISING ANTI-MRSA AGENTS

Authors

  • Petruta AELENEI “Grigore T. Popa” University of Medicine and Pharmacy Iasi, Romania
  • Cristina Elena HORHOGEA “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine of Iasi
  • Cristina Mihaela RIMBU Regulatory Affairs Department, Fiterman Pharma LLC, Iasi, Romania
  • G. DIMITRIU “Grigore T. Popa” University of Medicine and Pharmacy Iasi, Romania
  • Ana Clara APROTOSOAIE “Grigore T. Popa” University of Medicine and Pharmacy Iasi, Romania
  • Anca MIRON “Grigore T. Popa” University of Medicine and Pharmacy Iasi, Romania

Abstract

MORUSIN AND KUWANON G-PROMISING ANTI-MRSA AGENTS (Abstract): Synergy between natural products and antibiotics represent a promising strategy in the development of new antimicrobial therapies. Aim: The present work investigated possible synergistic interactions between prenylated flavones morusin and kuwanon G and antibiotics (tetracycline, ciprofloxacin) against methicillin-resistant Staphylococcus aureus (MRSA). Material and Methods: The minimum inhibitory concentrations (MICs) of prenylated flavones and antibiotics against MRSA (MRSA ATCC 33591 and four MRSA isolates of animal origin) were determined by the broth microdilution method. The interactions between prenylated flavones and antibiotics were evaluated by the checkerboard and time-kill assays. Results: In the broth microdilution assay, morusin and kuwanon G showed very good antibacterial activity (MICs ranging 3.13 and 12.50 µg/mL). The checkerboard assay demonstrated that morusin and kuwanon G act synergistically with both antibiotics; significant reductions in antibiotic MICs (2 to 512-fold) were recorded. The time-kill assay (at ½MIC) indicated bactericidal synergy for the combinations of morusin or kuwanon G with ciprofloxacin against S. aureus ATCC 33591. Conclusions: The combination of morusin or kuwanon G with antibiotics are good candidates for the development of novel treatments for MRSA infections in humans and animals.

Author Biographies

  • Petruta AELENEI, “Grigore T. Popa” University of Medicine and Pharmacy Iasi, Romania

    Faculty of Pharmacy,
    Department of Pharmaceutical Sciences (II)
    Regulatory Affairs Department, Fiterman Pharma LLC, Iasi, Romania

  • Cristina Elena HORHOGEA, “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine of Iasi

    Faculty of Veterinary Medicine
    Department of Public Health

  • G. DIMITRIU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi, Romania

    Faculty of Medicine
    Department of Preventive Medicine and Interdisciplinarity

  • Ana Clara APROTOSOAIE, “Grigore T. Popa” University of Medicine and Pharmacy Iasi, Romania

    Faculty of Pharmacy,
    Department of Pharmaceutical Sciences (II)

  • Anca MIRON, “Grigore T. Popa” University of Medicine and Pharmacy Iasi, Romania

    Faculty of Pharmacy,
    Department of Pharmaceutical Sciences (II)

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Additional Files

Published

2019-03-29