OPTIMIZATION OF PCR BASED ON DNA QUALITY IN ONCOLOGICAL RISK POPULATION DNA SAMPLES FROM BENIN

Authors

  • Sara Y. HOUNGUE University of Abomey Calavi (UAC), Benin
  • Haboubacar Elhadji Yaou MOUSSA University of Abomey Calavi (UAC), Benin
  • Venceslyne AGBAZAHOU University of Abomey Calavi (UAC), Benin
  • Aliona MORARIU “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania
  • Anca NEGURA “Alexandru Ioan Cuza” University of Iasi, Romania
  • Alina MANOLE “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania
  • Callinice D. CAPOCHICHI University of Abomey Calavi (UAC), Benin
  • Doina AZOICAI “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania
  • L. NEGURA “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania

Keywords:

BRCA1, BREAST CANCER, PROSTATE CANCER, MULTIPLEX-PCR

Abstract

In Benin, like other countries in the sub-Saharan region, breast cancer ranks first among all cancers. Material and methods: A highly heterogenic collection of 120 DNA samples from breast and prostate cancer patients and matched controls was available for further study on detection of BRCA1/2 gene mutations pattern. Results: Initially, the PCR amplification of BRCA1 exon 5 was performed for all samples, with poor results in terms of PCR efficiency. Only about a third of the samples showed visible bands of the expected size and with no contamination. A re-evaluation of DNA samples showed high variability on DNA concentration and quality, due to different extraction methods, sample origin and preservation condition. Conclusions: Our study primarily illustrate that DNA extracted from different tissue by different extraction methods may be damaged but still usable, by increasing the DNA amount in the PCR reactions and adjusting the other PCR parameters. However, appropriate caution should be exercised when analyzing samples derived from different tissue sources. By appropriate optimization, heterogenous samples can be simultaneously used for PCR amplifications with single parameters.

Author Biographies

  • Sara Y. HOUNGUE, University of Abomey Calavi (UAC), Benin

    Faculty of Sciences and Technics (FAST),
    Department of Biochemistry and Cell Biology
    Laboratory of Molecular Biomarkers in Cancer and Nutrition (BMCN)

  • Haboubacar Elhadji Yaou MOUSSA, University of Abomey Calavi (UAC), Benin

    Faculty of Sciences and Technics (FAST)
    Department of Biochemistry and Cell Biology
    Laboratory of Molecular Biomarkers in Cancer and Nutrition (BMCN)

  • Venceslyne AGBAZAHOU, University of Abomey Calavi (UAC), Benin

    Faculty of Sciences and Technics (FAST)
    Department of Biochemistry and Cell Biology
    Laboratory of Molecular Biomarkers in Cancer and Nutrition (BMCN)

  • Aliona MORARIU, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania

    Department of Oncogenetics

  • Anca NEGURA, “Alexandru Ioan Cuza” University of Iasi, Romania

    Department of Biology

  • Alina MANOLE, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania

    Department of Preventive Medicine and Interdisciplinarity

  • Callinice D. CAPOCHICHI, University of Abomey Calavi (UAC), Benin

    Faculty of Sciences and Technics (FAST),
    Department of Biochemistry and Cell Biology;
    Laboratory of Molecular Biomarkers in Cancer and Nutrition (BMCN),

  • Doina AZOICAI, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania

    Department of Oncogenetics
    Department of Preventive Medicine and Interdisciplinarity

  • L. NEGURA, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania

    Department of Oncogenetics

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Additional Files

Published

2022-12-27

Issue

Vol. 126 No. 4 (2022): The Medical-Surgical Journal

Section

PREVENTIVE MEDICINE - LABORATORY

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