EXPRESSION OF CYTOSKELETAL PROTEINS IN GLIOBLASTOMA CELL LINE

Authors

  • Elena Daniela SEMEN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • I. PRUTIANU “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • G. LUTA Regional Institute of Oncology, Iasi
  • Manuela CIOCOIU “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Keywords:

VIMENTIN, TRANSGELIN, CANCER, CELL CULTURE

Abstract

The overexpression of vimentin is linked to tumors with low differentiation, metastatic diseases, and reduced overall survival. The precise function of transgelin, an actin-binding protein, in the development of cancer is still debatable. Nevertheless, its participation in the mobility, differentiation and programmed cell death of malignant cells has been well-established. Material and methods: To investigate transgelin and transgelin expression, we used glioblastoma U87 cell line 2D culture. We conducted immunofluorescence staining to examine cultured cells. Results: the studied proteins were co-expressed in the studied cell line. Conclusions: How transgelin is expressed in cancerous cells is similar to the expression of vimentin. This similarity could suggest that transgelin may serve as an indicator of the level of aggression in cancer.

Author Biographies

  • Elena Daniela SEMEN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II)

  • I. PRUTIANU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (I)

  • G. LUTA, Regional Institute of Oncology, Iasi

    TRANSCEND Centre

  • Manuela CIOCOIU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II)

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Additional Files

Published

2023-06-30

Issue

Vol. 127 No. 2 (2023): The Medical-Surgical Journal

Section

BASIC SCIENCES

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