IN VITRO MEASUREMENT OF TOTAL ANTIOXIDANT CAPACITY OF CRATAEGUS MACRACANTHA LODD LEAVES
Abstract
Crataegus macracantha Lodd, family Rosaceae, is a very rare species in Europe, and unlike Crataegus monogyna is less investigated for pharmacologic activity. Aim: To analyze the ability of the lyophilisate of extract obtained from leaves of Crataegus macracantha Lodd (single plant at the Iaşi Botanical Garden) to capture free radicals in vitro. Material and methods: The lyophilisate obtained in Department of Pharmacognosy, Faculty of Pharmacy, "Grigore T. Popa" University of Medicine and Pharmacy Iaşi. The decreased absorbance of chromophore chlorpromazine radical cation in the presence of the lyophilized solutions was studied spectrophotometrically. The indicator radical cation, obtained by oxidation of chlorpromazine by potassium persulfate, has the maximum absorbance at 525 nm. Ascorbic acid was used as a standard antioxidant. Results: The absorbance of radical solution was determined after the addition of a certain amount of lyophilisate at different time intervals. The antioxidant activity was calculated using the calibration curve obtained by plotting the variation in radical solution absorbance depending on ascorbic acid concentration. For each ascorbic acid concentration the area under the curve was calculated from plotting the percentage inhibition of the absorbance at two pre-established time intervals. Conclusions: The results confirm the antioxidant activity of the leaves of Crataegus Macracantha Lodd and by optimizing the proposed analytical methods the antiradical activity can be quickly evaluated with minimal reagent consumption.
References
2. Proestos C, Lytoudi K, Mavromelanidou OK, Zoumpoulakis P, Sinanoglou JV. Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils. Antioxidants 2013; 2: 11-22.
3. Moon JK, Shibamoto T. Antioxidant assays for plant and food components. J Agric Food Chem 2009; 57(5):1655-1666.
4. Schlesier K, Harwat M, Böhm V, Bitsch R. Assessment of antioxidant activity by using different in vitro models. Free Radic Res 2002; 36(2):177-187.
5. Miftode AM, Stefanache A, Spac A, Dorneanu V. Spectrometric determination of total antioxidant activity in chlorpromazine radical cation - ascorbic acid system. Rev Med Chir Soc Med Nat Iaşi 2013; 117(3): 806-811.
6. Miftode AM, Stefanache A, Spac A, Dorneanu V. Evaluation of free radical scavenging activity of some antioxidants.Rev Med Chir Soc Med Nat Iaşi 2014; 118(1): 239-243.
7. Apak R, Gorinstein S, Böhm V et al. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure Appl Chem 2013; 85(5): 957-998.
8. Giurescu Bedreag CF, Trifan A, Vasincu Al, Miron SD, Aprotosoaie AC, Miron A. In vitro screening of Crataegus succulenta extracts for free radical scavenging and 15-lipoxygenase inhibitory activities. Rev Med Chir Soc Med Nat Iaşi 2014; 118(2): 544-550.
9. Robu S, Aprotosoaie AC, Miron A, Cioancă O, Stănescu U, Hâncianu M. In vitro antioxidant activity of ethanolic extracts from some Lavandula Species cultivated in Romania. Farmacia 2012; 60(3): 394-401.
Additional Files
Published
Issue
Section
License
COPYRIGHT
Once an article is accepted for publication, MSJ requests a transfer of copyrights for published articles.
COPYRIGHT TRANSFER FORM FOR
REVISTA MEDICO-CHIRURGICALĂ A SOCIETĂȚII DE MEDICI ȘI NATURALIȘTI DIN IAȘI /
THE MEDICAL-SURGICAL JOURNAL OF THE SOCIETY OF PHYSICIANS AND NATURALISTS FROM IASI
We, the undersigned authors of the manuscript entitled
_____________________________________________________________________________________
_____________________________________________________________________________________
warrant that this manuscript, which is submitted for publication in the REVISTA MEDICO-CHIRURGICALĂ, has not been published and it is not under consideration for publication in another journal.
- we give the consent for publication in the REVISTA MEDICO-CHIRURGICALĂ, in printed and electronic format and we transfer unconditioned and complete the copyright of this manuscript to the REVISTA MEDICO-CHIRURGICALĂ, in the event of its acceptance.
- the manuscript does not break the intellectual property rights of any other person.
- we have read the submitted version of the manuscript and we are fully responsible for the content.
Names and signatures of authors / copyright owners (the following sequence is the authorship of the article):
- ______________________________/_________________________
- ______________________________/_________________________
- ______________________________/_________________________
N.B. All the authors must sign this form
