CHEMICAL ASSESSMENT AND BIOLOGIC POTENTIAL OF A SPECIAL LESPEDEZA CAPITATA EXTRACT

Authors

  • Roxana-Delia CHITIALA “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Ana Flavia BURLEC “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Anisoara NISTOR Human Vanelli, VANELLI SRL, Iasi, Romania
  • Ioana Cezara CABA
  • Cornelia MIRCEA “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Monica HANCIANU “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Oana CIOANCA “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Abstract

Secondary metabolites are natural compounds synthesized by plants as an adaptation to environmental factors. Humans use such components for the prevention and treatment of minor or mild conditions. Lespedeza capitata is a medicinal and melliferous plant known for its various beneficial properties (antioxidant, antiaging, antiedematous, etc.). The objectives of this study were to identify and quantify the secondary metabolites from a selective L. capitata extract. Moreover, the evaluation of the antioxidant activity was part of the present research. Material and methods: Identification and semi-quantification were assessed by liquid chromatography (HPLC/MS), and the total flavonoids and polyphenols were determined by spectrophotometry according to European Pharmacopeia indications. DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and iron chelation test were used to establish the antioxidant putative effects of the investigated extract. Results: Among the identified compounds different quercetin derivatives, chlorogenic acid, and rosmarinic acid were found. The total polyphenols amounted to 174.24 mg/g GAE and flavonoids measured 95.65 mg/g rutoside equivalents. The antioxidant activity showed a good capacity to scavenge free radicals and a higher potential to chelate ferrous ions. The intensity of the effect was correlated to concentration. All in all, the investigated L. capitata extract demonstrated a polyphenol and flavonoid-rich content with good antioxidant potential.

Author Biographies

  • Roxana-Delia CHITIALA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Pharmacy
    Department of Pharmacognosy

  • Ana Flavia BURLEC, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Pharmacy
    Department of Pharmacognosy

  • Ioana Cezara CABA

    Faculty of Pharmacy
    Department of Toxicology

  • Cornelia MIRCEA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Pharmacy
    Department of Pharmaceutical Biochemistry and Clinical Laboratory

  • Monica HANCIANU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Pharmacy
    Department of Pharmacognosy

  • Oana CIOANCA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Pharmacy
    Department of Pharmacognosy

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Additional Files

Published

2023-09-30

Issue

Vol. 127 No. 3 (2023): The Medical-Surgical Journal

Section

PHARMACY

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