THE BENEFIT OF MOLECULAR MICROBIOLOGICAL ASSAYS IN OPTIMAL MONITORING OF PERIODONTAL DISEASE. A RETROSPECTIVE STUDY
Abstract
Periodontal disease is among the most frequent pathologies worldwide, with multiple consequences to the quality of daily patient life. Aim: To assess the prevalence and the DNA load of etiologic agents in selected patients with periodontal disease. Materials and methods: 55 saliva samples from periodontal disease patients were analyzed by multiplex Real Time PCR, using specific primers for: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola. The positive sampled were further analysed for the evaluation the antibiotic susceptibility to Clindamycin, Metronidazole, Amoxicillin, Doxycycline, Ciprofloxacin, and Clarithromycin. Results: The most frequent bacteria detected were Treponema denticola, in samples from 47 patients (85.5%), followed by Porphyromonas gingivalis, in 43 patients (78.2%) and Aggregatibacter actinomycetemcomitans, in 5 patients (9.1%). Besides these 3 periodontal pathogens, one patient was positive for Tannerella forsythia, Prevotella intermedia, Eubacterium nodatum and Capnocytophaga gingivalis. Conclusions: The microbiologic diagnosis using sensitive molecular assay is compulsory for modern and optimal periodontal diseases assessment.
References
2. West N, Chapple I, Claydon N, et al. BSP implementation of European S3 - level evidence-based treatment guidelines for stage I-III periodontitis in UK clinical practice. J Dent. 2021; 106: 103562.
3. Duncan HF, Kirkevang LL, Peters OA, et al. Treatment of pulpal and apical disease: The European Society of Endodontology (ESE) S3-level clinical practice guideline. Int Endod J. 2023; 56(Suppl 3): 238-295.
4. Herrera D, van Winkelhoff AJ, Matesanz P, Lauwens K, Teughels W. Europe’s contribution to the evaluation of the use of systemic antimicrobials in the treatment of periodontitis. Periodontol 2000 2023 / doi: 10.1111/prd.12492
5. Ergün E, Toraman E, Barış Ö, Budak H, Demir T. Quantitative investigation of the bacterial content of periodontal abscess samples by real-time PCR. J Microbiol Methods 2023; 213: 106826.
6. Reddahi S, Bouziane A, Dib K, Tligui H, Ennibi OK. qPCR Detection and Quantification of Aggre-gatibacter actinomycetemcomitans and Other Periodontal Pathogens in Saliva and Gingival Crevicular Fluid among Periodontitis Patients. Pathogens 2023; 12(1): 76.
7. 7.Chigasaki O, Aoyama N, et al. Porphyromonas gingivalis, the most influential pathogen in red-complex bacteria: A cross-sectional study on the relationship between bacterial count and clinical per-iodontal status in Japan. J Periodontol 2021; 92(12): 1719-1729.
8. Eick S, Pietkiewicz M, Sculean A. Oral microbiota in Swiss adolescents. Clin Oral Investig 2013; 17(1): 79-86.
9. Wang P, Duan D, Zhou X, et al. Relationship between expression of human gingival beta-defensins and levels of periodontopathogens in subgingival plaque. J Periodontal Res 2015; 50(1): 113-122.
10. Castillo Y, Delgadillo NA, Neuta Y, et al. Design and validation of a quantitative polymerase chain reaction test for the identification and quantification of uncultivable bacteria associated with perio-dontitis. Arch Oral Biol 2023; 154: 105758.
11. Ursu RG, Iancu LS, Porumb-Andrese E, et al. Host mRNA Analysis of Periodontal Disease Patients Positive for Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Tannerella for-sythia. Int J Mol Sci 2022;23(17): 9915.
12. Goriuc A, Cojocaru KA, Luchian I, Ursu RG, Butnaru O, Foia L. Using 8-Hydroxy-2′-Deoxiguanosine (8-OHdG) as a Reliable Biomarker for Assessing Periodontal Disease Associated with Diabetes. IJMS 2024; 25(3): 1425.
13. Tzanetakis GN, Koletsi D, Tsakris A, Vrioni G. Prevalence of Fungi in Primary Endodontic Infections of a Greek-living Population Through Real-time Polymerase Chain Reaction and Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry. J Endod. 2022; 48(2): 200-207.
14. Rams TE, Sautter JD, Getreu A, van Winkelhoff AJ. Phenotypic identification of Porphyromonas gingivalis validated with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Microb Pathog 2016; 94: 112-116.
15. Jeong J, Oh Y, Jeon J, et al. Effective microbial molecular diagnosis of periodontitis-related pathogen Porphyromonas gingivalis from salivary samples using rgpA gene. Genomics Inform. 2022; 21(1): e13.
Additional Files
Published
Issue
Section
License
COPYRIGHT
Once an article is accepted for publication, MSJ requests a transfer of copyrights for published articles.
COPYRIGHT TRANSFER FORM FOR
REVISTA MEDICO-CHIRURGICALĂ A SOCIETĂȚII DE MEDICI ȘI NATURALIȘTI DIN IAȘI /
THE MEDICAL-SURGICAL JOURNAL OF THE SOCIETY OF PHYSICIANS AND NATURALISTS FROM IASI
We, the undersigned authors of the manuscript entitled
_____________________________________________________________________________________
_____________________________________________________________________________________
warrant that this manuscript, which is submitted for publication in the REVISTA MEDICO-CHIRURGICALĂ, has not been published and it is not under consideration for publication in another journal.
- we give the consent for publication in the REVISTA MEDICO-CHIRURGICALĂ, in printed and electronic format and we transfer unconditioned and complete the copyright of this manuscript to the REVISTA MEDICO-CHIRURGICALĂ, in the event of its acceptance.
- the manuscript does not break the intellectual property rights of any other person.
- we have read the submitted version of the manuscript and we are fully responsible for the content.
Names and signatures of authors / copyright owners (the following sequence is the authorship of the article):
- ______________________________/_________________________
- ______________________________/_________________________
- ______________________________/_________________________
N.B. All the authors must sign this form