THE BENEFIT OF MOLECULAR MICROBIOLOGICAL ASSAYS IN OPTIMAL MONITORING OF PERIODONTAL DISEASE. A RETROSPECTIVE STUDY

Authors

  • G. NICHITEAN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • I. LUCHIAN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Monica Silvia TATARCIUC “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Oana BEJAN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Simona GIUSCA “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Ancuta GORIUC “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • C. DAMIAN “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Irina-Draga CARUNTU “Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Ramona Gabriela URSU “Grigore T. Popa” University of Medicine and Pharmacy Iasi

Abstract

Periodontal disease is among the most frequent pathologies worldwide, with multiple consequences to the quality of daily patient life. Aim: To assess the prevalence and the DNA load of etiologic agents in selected patients with periodontal disease. Materials and methods: 55 saliva samples from periodontal disease patients were analyzed by multiplex Real Time PCR, using specific primers for: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola. The positive sampled were further analysed for the evaluation the antibiotic susceptibility to Clindamycin, Metronidazole, Amoxicillin, Doxycycline, Ciprofloxacin, and Clarithromycin. Results: The most frequent bacteria detected were Treponema denticola, in samples from 47 patients (85.5%), followed by Porphyromonas gingivalis, in 43 patients (78.2%) and Aggregatibacter actinomycetemcomitans, in 5 patients (9.1%). Besides these 3 periodontal pathogens, one patient was positive for Tannerella forsythia, Prevotella intermedia, Eubacterium nodatum and Capnocytophaga gingivalis. Conclusions: The microbiologic diagnosis using sensitive molecular assay is compulsory for modern and optimal periodontal diseases assessment.

Author Biographies

  • G. NICHITEAN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Dental Medicine
    Department of Dental Technology

  • I. LUCHIAN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Dental Medicine
    Department of Periodontology

  • Monica Silvia TATARCIUC, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Dental Medicine
    Department of Dental Technology

  • Oana BEJAN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Dental Medicine
    Department of Orthodontics

  • Simona GIUSCA, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-functional Sciences (I)

  • Ancuta GORIUC, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Dental Medicine
    Department of Biochemistry

  • C. DAMIAN, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Preventive Medicine and Interdisciplinarity

  • Irina-Draga CARUNTU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-functional Sciences (I)

  • Ramona Gabriela URSU, “Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Preventive Medicine and Interdisciplinarity

References

1. Chen MX, Zhong YJ, Dong QQ, Wong HM, Wen YF. Global, regional, and national burden of severe periodontitis, 1990-2019: An analysis of the Global Burden of Disease Study 2019. J Clinic Perio-dontology 2021; 48(9): 1165-1188.
2. West N, Chapple I, Claydon N, et al. BSP implementation of European S3 - level evidence-based treatment guidelines for stage I-III periodontitis in UK clinical practice. J Dent. 2021; 106: 103562.
3. Duncan HF, Kirkevang LL, Peters OA, et al. Treatment of pulpal and apical disease: The European Society of Endodontology (ESE) S3-level clinical practice guideline. Int Endod J. 2023; 56(Suppl 3): 238-295.
4. Herrera D, van Winkelhoff AJ, Matesanz P, Lauwens K, Teughels W. Europe’s contribution to the evaluation of the use of systemic antimicrobials in the treatment of periodontitis. Periodontol 2000 2023 / doi: 10.1111/prd.12492
5. Ergün E, Toraman E, Barış Ö, Budak H, Demir T. Quantitative investigation of the bacterial content of periodontal abscess samples by real-time PCR. J Microbiol Methods 2023; 213: 106826.
6. Reddahi S, Bouziane A, Dib K, Tligui H, Ennibi OK. qPCR Detection and Quantification of Aggre-gatibacter actinomycetemcomitans and Other Periodontal Pathogens in Saliva and Gingival Crevicular Fluid among Periodontitis Patients. Pathogens 2023; 12(1): 76.
7. 7.Chigasaki O, Aoyama N, et al. Porphyromonas gingivalis, the most influential pathogen in red-complex bacteria: A cross-sectional study on the relationship between bacterial count and clinical per-iodontal status in Japan. J Periodontol 2021; 92(12): 1719-1729.
8. Eick S, Pietkiewicz M, Sculean A. Oral microbiota in Swiss adolescents. Clin Oral Investig 2013; 17(1): 79-86.
9. Wang P, Duan D, Zhou X, et al. Relationship between expression of human gingival beta-defensins and levels of periodontopathogens in subgingival plaque. J Periodontal Res 2015; 50(1): 113-122.
10. Castillo Y, Delgadillo NA, Neuta Y, et al. Design and validation of a quantitative polymerase chain reaction test for the identification and quantification of uncultivable bacteria associated with perio-dontitis. Arch Oral Biol 2023; 154: 105758.
11. Ursu RG, Iancu LS, Porumb-Andrese E, et al. Host mRNA Analysis of Periodontal Disease Patients Positive for Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Tannerella for-sythia. Int J Mol Sci 2022;23(17): 9915.
12. Goriuc A, Cojocaru KA, Luchian I, Ursu RG, Butnaru O, Foia L. Using 8-Hydroxy-2′-Deoxiguanosine (8-OHdG) as a Reliable Biomarker for Assessing Periodontal Disease Associated with Diabetes. IJMS 2024; 25(3): 1425.
13. Tzanetakis GN, Koletsi D, Tsakris A, Vrioni G. Prevalence of Fungi in Primary Endodontic Infections of a Greek-living Population Through Real-time Polymerase Chain Reaction and Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry. J Endod. 2022; 48(2): 200-207.
14. Rams TE, Sautter JD, Getreu A, van Winkelhoff AJ. Phenotypic identification of Porphyromonas gingivalis validated with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Microb Pathog 2016; 94: 112-116.
15. Jeong J, Oh Y, Jeon J, et al. Effective microbial molecular diagnosis of periodontitis-related pathogen Porphyromonas gingivalis from salivary samples using rgpA gene. Genomics Inform. 2022; 21(1): e13.

Additional Files

Published

2024-03-29