ATHEROGENIC INDEX OF PLASMA (AIP): CAN BE A BIOMARKER FOR EARLY ENDOTHELIAL DYSFUNCTION AND CARDIOVASCULAR DISEASE IN EXPERIMENTAL MODELS OF OBESITY RATS?

Authors

  • Andreea CLIM Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Minela Aida MARANDUCA Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • A.C. PANZARIU Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Ludmila LOZNEANU Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • A. ALEXANDRU “Victor Babes” University of Medicine and Pharmacy Timisoara, Romania
  • I.F. FAUR “Victor Babes” University of Medicine and Pharmacy Timisoara, Romania
  • R.A. BAISAN Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine Iași
  • A. SZILAGYI Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • B.I. TAMBA Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • D.N. ȘERBAN Grigore T. Popa” University of Medicine and Pharmacy Iasi
  • Ionela Lacramioara SERBAN Grigore T. Popa” University of Medicine and Pharmacy Iasi

Abstract

Objective: Our experimental study evaluated the influence and correlation among the Atherogenic index of plasma and early histological endothelial dysfunction in an experimental model of obesity rats. Materials and methods: Twenty-one Wistar rats, male, aged 4–6 months (450-550g), were allocated into III groups. The control group consumed exclusively a standard food, while the II group was administered HFD (Hight-Fat-Diet) with 2% cholesterol and the last group received HFD and treatment with Atorvastatin 20mg/kg/day by gavages. Before and after 4 weeks of oral administration of diet and treatment, serum lipid profile and glycemia were measured. The Atherogenic Index of Plasma, Castelli risk indices (I and II) and lipoprotein combined indices (LCI) are evaluated with a formula. Also, for histological study, aortas were extracted for the histological investigation and the principal extracellular matrix components, including collagen and elastin, were examined with a microscope for indications of aortic degeneration. Endothelial dysfunction was evaluated with biochemical levels of lipid profile, abdominal ultrasonography and histological study. Results: Four-week diet supplementation with HFD for obesity rats caused an increase in serum lipids levels; AIP levels indicate a high risk for cardiovascular events, histological aspect we observed endothelial damage, with degenerative modification in the aortic media, indicating by the dissociation of elastic fibers and accumulation of collagen. Conclusions: In our study, AIP, lipoprotein combined index and Castelli’s risk index I and II are correlated with endothelial injury. Obesity disturbs the lipid profile, increasing AIP in experimental models of obesity rats and can be a risk factor for endothelial dysfunction. Treatment with statin, healthy food and change in the modifiable risk factors can improve endothelial function and early stage of atherosclerosis.

Author Biographies

  • Andreea CLIM, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II) - Division of Physiology

  • Minela Aida MARANDUCA, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II) - Division of Physiology
    “Sf. Spiridon” County Clinical Emergency Hospital Iasi, Romania
    Internal Medicine Clinic

  • A.C. PANZARIU, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II) - Division of Physiology

  • Ludmila LOZNEANU, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (I) - Division of Histology

  • A. ALEXANDRU, “Victor Babes” University of Medicine and Pharmacy Timisoara, Romania

    Faculty of Medicine / Department of General Surgery

  • I.F. FAUR, “Victor Babes” University of Medicine and Pharmacy Timisoara, Romania

    Faculty of Medicine / Department of General Surgery
    Emergency County Hospital, Timisoara, Romania / IInd Surgery Clinic

  • R.A. BAISAN, Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine Iași

    Faculty of Veterinary Medicine / Department of Clinical Sciences

  • A. SZILAGYI, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Advanced Research and Development Center in Experimental Medicine (CEMEX)

  • B.I. TAMBA, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Advanced Research and Development Center in Experimental Medicine (CEMEX)

  • D.N. ȘERBAN, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II) - Division of Physiology

  • Ionela Lacramioara SERBAN, Grigore T. Popa” University of Medicine and Pharmacy Iasi

    Faculty of Medicine
    Department of Morpho-Functional Sciences (II) - Division of Physiology

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Additional Files

Published

2024-06-28