MESENCHYMAL STEM CELL’S OSTEOGENIC DIFFERENTIATION IS DEPENDENT ON ENVIRONMENTAL STIMULI

Authors

  • C. I. CRETU ‟Grigore Popa” University of Medicine and Pharmacy Iași
  • Andreea TIBEICA IORDAN ‟Grigore Popa” University of Medicine and Pharmacy Iași
  • S. C. TIBEICA ‟Grigore Popa” University of Medicine and Pharmacy Iași
  • Norina Consuela FORNA ‟Grigore Popa” University of Medicine and Pharmacy Iași
  • M. COSTULEANU ‟Grigore Popa” University of Medicine and Pharmacy Iași

DOI:

https://doi.org/10.22551/MSJ.2024.04.16

Abstract

Mesenchymal stem cells derived osteoblasts or mesenchymal stem cells osteogenic differentiation could represent an important way to increase the healing after dental implantation. Aim: This research investigated the effects of various environmental stimuli (in vitro) on the osteogenic differentiation of MSCs and the subsequent development of calcium deposits by these cells. Materials and methods: Calcium phosphate deposition was quantified using the Alizarin red method via spectrophotometry. Results: The calcium deposited solely by mesenchymal stem cells derived osteoblasts was considered to be 100% all the time. When 1% platelet-rich plasma was added in the culture medium, the calcium deposits increased by 20% (on average). Different amounts of calcium deposits were developed by MSCs-derived osteoblasts when platelet-rich plasma was administered concurrently with: rosavin, rapamycin, metformin, “young” exosomes, “adult” exosomes, lower power CO2 laser, extracellular shock waves, and PI3K/HDAC11 inhibitor. Conversely, platelet-poor plasma did not significantly modify the calcium deposits realized by MSCs-derived osteoblasts. Conclusions: All the obtained data suggest that calcium deposits resulting from the functioning of mesenchymal stem cells-derived osteoblasts could be significantly and especially increased by cellular mechanisms related to “young” exosomes, rosavin, acetylation, rapamycin, “adult” exosomes and metformin. 

Author Biographies

  • C. I. CRETU, ‟Grigore Popa” University of Medicine and Pharmacy Iași

    Faculty of Dental Medicine
    Department of Implantology, Removable Prostheses, Dental Prostheses Technology

  • Andreea TIBEICA IORDAN, ‟Grigore Popa” University of Medicine and Pharmacy Iași

    Faculty of Dental Medicine
    Department of Implantology, Removable Prostheses, Dental Prostheses Technology

  • S. C. TIBEICA, ‟Grigore Popa” University of Medicine and Pharmacy Iași

    Faculty of Dental Medicine
    Department of Implantology, Removable Prostheses, Dental Prostheses Technology

  • Norina Consuela FORNA, ‟Grigore Popa” University of Medicine and Pharmacy Iași

    “Grigore T. Popa” University of Medicine and Pharmacy Iasi
    Faculty of Dental Medicine
    Department of Implantology, Removable Prostheses, Dental Prostheses Technology

  • M. COSTULEANU, ‟Grigore Popa” University of Medicine and Pharmacy Iași

    Faculty of Dental Medicine
    Department of Physiopathology

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Additional Files

Published

2024-12-23

Issue

Vol. 128 No. 4 (2024): The Medical-Surgical Journal

Section

BASIC SCIENCES

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