INTRAOCULAR BIODISTRIBUTION OF INTRAVITREAL INJECTED FLUORESCENT DEXAMETHASONE-CHITOSAN NANOPARTICLES IN RABBIT EYES

Authors

  • Andreea Dana MORARU University of Medicine and Pharmacy“Grigore T. Popa” – Iaşi
  • M. COSTULEANU University of Medicine and Pharmacy“Grigore T. Popa” – Iaşi
  • D. COSTIN University of Medicine and Pharmacy“Grigore T. Popa” – Iaşi
  • Catalina PEPTU “Gheorghe Asachi” Technical University, Iaşi
  • M. POPA “Gheorghe Asachi” Technical University, Iaşi
  • D. CHISELITA University of Medicine and Pharmacy“Grigore T. Popa” – Iaşi

Abstract

Aim: To evaluate intraocular biodistribution of fluorescent nanoparticles composed of dexamethasone bound to chitosan after intravitreal administration in rabbit eyes. Material and methods: The chitosan and gelatin based nanoparticles were synthetized using a reverse emulsion-double crosslinking technique (ionic and covalent) and then dexamethasone was bound. Two units of 1% suspension of these nanoparticles in saline solution were injected intravitreally into rabbit eyes. The histologic sections obtained at 72 hours were analyzed by confocal microscopy. Results: The chitosan-fluorescein conjugate bound to dexamethasone was present in all ocular tissues at 72 hours. The nanoparticles were present in the retina and lens in a larger amount than in the other ocular tissues. Conclusions: The reverse emulsion-double crosslinking technique was efficient in synthesizing a biocompatible polymeric nanosystem. The in vivo study of intraocular biodistribution of fluorescein-marked nanoparticles capable of binding dexamethasone revealed their affinity for the retina and lens after intravitreal administration.

Author Biographies

  • Andreea Dana MORARU, University of Medicine and Pharmacy“Grigore T. Popa” – Iaşi

    Faculty of Medicine
    Departament of Surgery

  • M. COSTULEANU, University of Medicine and Pharmacy“Grigore T. Popa” – Iaşi

    Faculty of Dental Medicine
    Departament of Surgery

  • D. COSTIN, University of Medicine and Pharmacy“Grigore T. Popa” – Iaşi

    Faculty of Medicine
    Departament of Surgery

  • Catalina PEPTU, “Gheorghe Asachi” Technical University, Iaşi

    Faculty of Chemical Engineering and Environmental Protection
    Department of Natural and Synthetic Polymers

  • M. POPA, “Gheorghe Asachi” Technical University, Iaşi

    Faculty of Chemical Engineering and Environmental Protection
    Department of Natural and Synthetic Polymers

  • D. CHISELITA, University of Medicine and Pharmacy“Grigore T. Popa” – Iaşi

    Faculty of Medicine
    Departament of Surgery

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Additional Files

Published

2015-06-30

Issue

Vol. 119 No. 2 (2015): The Medical-Surgical Journal

Section

BASIC SCIENCES

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