EFFECTS OF ZINGIBER OFFICINALE ROSCOE FRESH EXTRACT ON AMELANOTIC MELANOMA AND NORMAL SKIN FIBROBLASTS

Authors

  • Sabina Ioana COJOCARU University „Alexandru Ioan Cuza” - Iași
  • Miruna STAN University of Bucharest - Bucharest
  • G. STOIAN University of Bucharest - Bucharest
  • Anca DINISCHIOTU University of Bucharest - Bucharest

Abstract

Zingiber officinale Roscoe is a very important medicinal plant, with a long history of therapeutic uses, especially in oriental traditional medicine. Aim: To investigate the cytotoxicity of a fresh ginger extract on some skin tumor cells compared to normal cells. Material and methods: C32 amelanotic melanoma cell line and CCD human skin fibroblasts were used. The fresh extract obtained by crushing ginger rhizome was examined for phenolic content. The in vitro cytotoxicity was examined using phase contrast microscopy and MTT assay for the concentrations of 2 and 4 mg% total phenols. Results: Both concentrations used for treatment induced no changes in normal the morphology and viability of fibroblasts compared to control cells. Amelanotic melanoma cells displayed profound changes in cell morphology such as cell shrinkage, rounding up and membrane blebbing and a decrease in cell viability in a dose-dependent manner. Conclusions: Fresh ginger extract induced no changes in normal skin fibroblast viability, but caused profound cytotoxic effects on amelanotic melanoma. These results could encourage further studies regarding the intimate mechanisms of the antitumor action displayed by the fresh ginger extract.

Author Biographies

  • Sabina Ioana COJOCARU, University „Alexandru Ioan Cuza” - Iași

    Faculty of Biology
    Department of Biology

  • Miruna STAN, University of Bucharest - Bucharest

    Faculty of Biology
    Department of Biochemistry and Molecular Biology

  • G. STOIAN, University of Bucharest - Bucharest

    Faculty of Biology
    Department of Biochemistry and Molecular Biology

  • Anca DINISCHIOTU, University of Bucharest - Bucharest

    Faculty of Biology
    Department of Biochemistry and Molecular Biology

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Additional Files

Published

2015-06-30

Issue

Vol. 119 No. 2 (2015): The Medical-Surgical Journal

Section

PHARMACY

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