RESEARCH ON PLASMA AND SALIVA LEVELS OF SOME BIVALENT CATIONS IN PATIENTS WITH CHRONIC PERIODONTITIS (SALIVARY CATIONS IN CHRONIC PERIODONTITIS)
Keywords:
CHRONIC PERIODONTITIS, CALCIUM, MAGNESIUM, ZINC, COPPER.Abstract
The purpose of this study was to determine whether chronic periodontitis can stand behind modifications in the salivary and blood concentration of some bivalent cations (Calcium, Magnesium, Zinc and Copper). For this purpose, we formed a group of 30 adult patients with clinically onset chronic periodontitis, and another one of 30 healthy patients as control. Both groups were free from acute oral pathology and general illnesses. The groups were divided again according to the habit of smoking. Total saliva samples were obtained as “first time in the morning”, then weighed and processed. Cations were read on Atomic Absorption Spectrophotometer and by Ion Chromatography (Magnesium). The same patients were required to undergo laboratory blood tests for Calcium, Magnesium and Zinc. Data obtained was normalised, then statistically interpreted using two-tailed heteroscedastic t-Student tests. Our data confirmed the existence of a connection between salivary calcium, magnesium, zinc and copper, and of blood magnesium, and chronic periodontitis. Salivary calcium and magnesium are affected by smoking.
References
2. Löe H, Theilande E, Jensen SB, Experimental gingivitis in man. J. Periodontol. 1965, 36: 177-187.
3. Genco RJ, Host responses in periodontal diseases: current concepts. J. Periodontol. 1992; 63: 338-355.
4. Heitz-Mayfield LJA, Disease progression: identification of high-risk groups and individuals for peri-odontitis. J. Clin. Periodontol. 2005; 32: 196-209.
5. Seymour GJ, Importance of the host response in the periodontium. J. Clin. Periodontol. 1991; 18: 421-426.
6. Alarcón OM, Burguera JL, Burguera M, Suárez N, Reinosa J, Changes in copper, zinc and iron content in serum and saliva in various otorhinolaryngological ailments, J Trace Elem Electrolytes Health Dis. 1989; 3(4): 203-208.
7. Büntzel J, Bruns F, Glatzel M, Garayev A, Mücke R, Kisters K, et al. Zinc concentrations in serum during head and neck cancer progression, Anticancer Res. 2007; 27 (4A): 1941-1943.
8. Gradinaru, I; Ghiciuc, CM; Popescu, E; Nechifor, C; Mandreci, I; Nechifor, M; Blood plasma and saliva levels of magnesium and other bivalent cations in patients with parotid gland tumors, Magnes Res, 2007; 20(4): 254-258.
9. Henkin RI, Gouliouk V, Fordyce A, Arch Oral Biol. 2012; 57(2): 205-210. Erratum in: Arch Oral Biol. 2013; 58(1): 110.
10. Page RC, Schroeder HE, Pathogenesis of chronic inflammatory periodontal disease. A summary of current work. Lab Invest 1976; 34: 235-249.
11. Seymour GJ, Powell RN, Cole KL, Aitken JF, Brooks D, Bradely I, et al., Experimental gingivitis in humans. A histochemical and immunological characterization of the lymphoid cell subpopulations.. J. Periodontal Res. 1983; 18: 375-385.
12. Seymour GJ, Gemmell E, Walsh LJ, Powell RN, Immuno-histological analysis of experimental gin-givitis in humans. Clin. Exp. Immunol. 1988; 71: 132-137.
13. Lindhe J, Rylander H, Experimental gingivitis in young dogs. Scand. J. Dent. Res. 1975; 83: 314-326.
14. Berglundh T, Donati M, Aspects of adaptive host response in periodontitis. J. Clin. Periodontol. 2005; 32: 87-107.
15. Poulter LW, Seymour GJ, Duke O, Panayi G, Janossy G, Immunohistological analysis of delayed-type hypersensitivity in man. Cell. Imunol. 1982; 74: 358-369.
16. Heidermann D., Parodontologie, Elsevier, Urban & Fischer Verlag, 2005
17. Bales, CW; Freeland-Graves, JH; Askey, S; Behmardi, F; Pobocik, RS; Fickel, JF; et al; Zinc, magne-sium, copper, and protein concentrations in human saliva: age- and sex-related differences; Am J Clin Nutr 1990; 51: 462-469
18. Rossol, M; Pierer, M; Raulien, N; Quandt, D; Meusch, U; Rothe, K; et al., Extracellular Ca(2+) is a danger signal activating the NLRP3 inflammasome through G protein-coupled calcium sensing recep-tors, Nat Commun (2012), PMID 23271661
19. David, B; Rosalind, A; Le Feuvre, RD; Wheeler, NS; Hilfiker, S; Rothwell, NJ; et al; Ca 2+ Stores and Ca2+ Entry Differentially Contribute to the Release of IL-1 from Murine Macrophages, J Immunol, 2003; 2: 172
20. Stober, CB; Lammas, DA; Cheuk, ML; Dinikantha, S; Kumararatne, S; Lightman, SL; McArdle, CA., ATP-mediated killing of Mycobacterium bovis bacille calmette-Guerin within human macrophages is calcium dependent and associated with the acidification of mycobacteria-containing phagosomes., Journal of Immunology, 2001; 166: 6276-6286
21. Kuraner T, Sinan Beksac M, Kayakirilmaz K, Caĝlayan F, Önderoĝlu LS, Özgünes H, Serum and parotid saliva testosterone, calcium, magnesium, and zinc levels in males, with and without periodon-titis, Biological Trace Element Research, 1991; 31(1): 43-49.
22. Sugimoto J, Romani AM, Valentin-Torres AM, Luciano AA, Ramirez Kitchen CM, Funderburg N, et al., Magnesium decreases inflammatory cytokine production: a novel innate immunomodulatory mechanism, J Immunol. 2012; 188 (12): 6338-6346.
23. Rochelson B, Dowling O, Schwartz N, Metz CN, Magnesium sulfate suppresses inflammatory re-sponses by human umbilical vein endothelial cells (HuVECs) through the NFkappaB pathway, J Re-prod Immunol. 2007; 73 (2): 101-107.
24. Mazur A, Maier JA, Rock E, Gueux E, Nowacki W, Rayssiguier Y, Magnesium and the inflammatory response: potential physiopathological implications, Arch Biochem Biophys. 2007; 458(1): 48-56.
25. Dowling O, Chatterjee PK, Gupta M, Tam Tam HB, Xue X, Lewis D, et al., Magnesium sulfate reduces bacterial LPS-induced inflammation at the maternal-fetal interface, Placenta. 2012; 33(5): 392-398.
26. Bussière FI, Gueux E, Rock E, Girardeau JP, Tridon A, Mazur A, et al., Increased phagocytosis and production of reactive oxygen species by neutrophils during magnesium deficiency in rats and inhibition by high magnesium concentration, Br J Nutr. 2002; 87(2): 107-113.
27. Pan HC, Sheu ML, Su HL, Chen YJ, Chen CJ, Yang DY, et al., Magnesium supplement promotes sciatic nerve regeneration and down-regulates inflammatory response, Magnes Res. 2011; 24(2): 54-70.
28. Weglicki WB, Hypomagnesemia and inflammation: clinical and basic aspects, Ann Rev Nutr. 2012 21; 32: 55-71.
29. Zhang C, Lu X, Tan Y, Li B, Miao X, Jin L et al, Diabetes-induced hepatic pathogenic damage, in-flammation, oxidative stress, and insulin resistance was exacerbated in zinc deficient mouse model, PLoS One. 2012; 7(12): e49257.
30. Shih CJ, Chiou YL, Zinc Sulfate Inhibited Inflammation of Der p2-Induced Airway Smooth Muscle Cells by Suppressing ERK1/2 and NF-κB Phosphorylation, Inflammation. 2012, 20. (Epub ahead of print)
31. Guo CH, Wang CL, Effects of zinc supplementation on plasma copper/zinc ratios, oxidative stress, and immunological status in hemodialysis patients, Int J Med Sci. 2013; 10(1): 79-89.
32. Kiss E, Sewon L, Gorzó I, Nagy K, Salivary calcium concentration in relation to periodontal health of female tobacco smokers: a pilot study, Quintessence Int. 2010; 41(9): 779-785.
33. Sutej I, Peros K, Benutic A, Capak K, Basic K, Rosin-Grget K, Salivary calcium concentration and periodontal health of young adults in relation to tobacco smoking, Oral Health Prev Dent. 2012; 10(4): 397-403
34. Kolte AP, Kolte RA, Laddha RK, Effect of smoking on salivary composition and periodontal status. J Indian Soc Periodontol 2012; 16(3): 350-353
35. Zuabi O, Machtei EE, Ben-Aryeh H, Ardekian L, Peled M, Laufer D. The ef¬fect of smoking and periodontal treatment on salivary composition in patients with established periodontitis. J Periodon-tol 1999; 70 (10): 1240-1246.

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