ANTIOXIDANT ACTIVITIES AND PHENOLIC CONTENTS OF EXTRACTS FROM GLINUS OPPOSITIFOLIUS AERIAL PARTS
Abstract
In Malian traditional medicine, aerial parts of Glinus oppositifolius are used in the treatment of many disorders, particularly inflammations and joint pains. Aim: The present study was initiated to investigate the antioxidant potential of Glinus oppositifolius. Material and methods: Aqueous and ethanol extracts were prepared from the dried aerial parts. Both extracts were examined for the phenolic content and antioxidant activity. Total phenolic content was quantified by Folin-Ciocalteu method. Antioxidant activity was studied in vitro by evaluating the ability to scavenge different free radicals (ABTS, superoxide and hydroxyl radicals, nitric oxide) and to chelate ferrous ions. Results: Aqueous extract was found to contain a higher level of total phenols than ethanol extract (1.27±0.04 vs. 0.94±0.05 g GAE/100 g extract). Both extracts lacked the ability to scavenge superoxide anion radical but scavenged ABTS and hydroxyl radicals, nitric oxide and chelated ferrous ions in a concentration-dependent manner. According to the EC50 values, aqueous extract showed stronger antioxidant effects than ethanol extract (174.32±1.49 vs. 276.76±1.52 µg/mL in ABTS radical cation scavenging assay, 119.91±1.70 vs. 240.57±0.97 µg/mL in nitric oxide scavenging assay, 1.99±0.01 vs. 6.54±0.08 mg/mL in ferrous ion chelating assay). In hydroxyl radical scavenging assay, aqueous extract had an EC50 value of 0.86±0.00 mg/mL while the EC50 value of ethanol extract was higher than the highest concentration that was tested (3.75 mg/mL). Conclusions: Our results clearly indicate the antioxidant potential of Glinus oppositifolius aerial parts and suggest that the anti-inflammatory activity might be partly related to the antioxidant potential.
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